Cell Transport

Master diffusion, osmosis, and active transport mechanisms for GCSE Biology.

# Cell Transport

Substances need to move in and out of cells for the organism to survive. There are three main transport mechanisms: diffusion, osmosis, and active transport.


1. Diffusion

The net movement of particles from an area of higher concentration to an area of lower concentration (down the concentration gradient).

  • Passive (no energy required)
  • Continues until equilibrium is reached
  • Examples: oxygen into cells, carbon dioxide out of cells, glucose absorption in the gut

Factors Affecting Rate of Diffusion

  • Concentration gradient: steeper → faster
  • Temperature: higher → faster (more kinetic energy)
  • Surface area: larger → faster
  • Membrane thickness: thinner → faster

2. Osmosis

The movement of water molecules from a dilute solution (high water concentration) to a concentrated solution (low water concentration) through a partially permeable membrane.

  • Special case of diffusion (water only)
  • Passive process

Effects on Cells

Solution Animal Cell Plant Cell
Hypotonic (dilute) Swells, may burst (lysis) Turgid (firm)
Isotonic Normal Normal
Hypertonic (concentrated) Shrinks (crenation) Plasmolysed (flaccid)

3. Active Transport

The movement of particles from a lower concentration to a higher concentration (against the concentration gradient) using energy from respiration.

  • Requires ATP (energy from mitochondria)
  • Uses carrier proteins in the cell membrane

Examples:

  • Root hair cells absorb mineral ions from soil
  • Gut cells absorb glucose even when concentration inside is higher

4. Comparing Transport Methods

Feature Diffusion Osmosis Active Transport
Direction High → Low High water → Low water Low → High
Energy needed? No No Yes (ATP)
What moves? Any small molecule Water only Specific molecules/ions
Carrier proteins? No No (through membrane) Yes

5. Required Practical: Osmosis

Investigate osmosis in potato cylinders:

  1. Cut potato into equal cylinders
  2. Place in different concentrations of sucrose solution
  3. Measure mass before and after (24 hours)
  4. Calculate percentage change in mass
  5. Plot graph of % change vs concentration

Where the line crosses 0% change = isotonic concentration.


6. Practice Questions

    1. Explain why osmosis is important for plants.
    1. Give two examples of active transport in the body.
    1. A plant cell is placed in pure water. Describe and explain what happens.
    1. Why do root hair cells have many mitochondria?

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Summary

  • Diffusion: high → low concentration; passive; any molecule
  • Osmosis: water through partially permeable membrane; dilute → concentrated; passive
  • Active transport: low → high; requires ATP; uses carrier proteins
  • Root hair cells and gut lining use active transport

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